Designing a turn-on ultrasensitive fluorescent probe based on ICT-FRET for detection and bioimaging of Hypochlorous acid

Hypochlorous acid (HClO) plays an essential role in biological systems. The characteristics of potent oxidization and short lifetime make it challenging to detect specifically from other reactive oxygen species (ROS) at cellular levels. Therefore, its detection and imaging with high selectivity and sensitivity are of great significance. Herein a turn-on HClO fluorescent probe (named RNB-OCl) with boronate ester as the recognition site was designed and synthesized. The RNB-OCl displayed good selective and ultrasensitive to HClO with a low detection limit of 1.36 nM by the intramolecular charge transfer (ICT)-fluorescence resonance energy transfer (FRET) dual mechanism in reducing the fluorescence background and improving the sensitivity. In addition, the role of the ICT-FRET was further demonstrated by time-dependent density functional theory (TD-DFT) calculations. Furthermore, the probe RNB-OCl was successfully employed for imaging HClO in living cells.