漆酶
吸光度
芦丁
检出限
化学
鸟苷
纳米棒
比色法
肉眼
离子键合
离子强度
色谱法
纳米技术
材料科学
生物化学
有机化学
抗氧化剂
酶
离子
水溶液
作者
Kowsar Davoodi-Rad,Ardeshir Shokrollahi,Faezeh Shahdost-fard,Kamal Azadkish
出处
期刊:Biosensors
[MDPI AG]
日期:2023-03-12
卷期号:13 (3): 374-374
被引量:5
摘要
Inspired by laccase activity, herein, Cu-guanosine nanorods (Cu-Guo NRs) have been synthesized for the first time through a simple procedure. The activity of the Cu-Guo NR as the laccase mimicking nanozyme has been examined in the colorimetric sensing of rutin (Rtn) by a novel and simple spectrophotometric method. The distinct changes in the absorbance signal intensity of Rtn and a distinguished red shift under the optimum condition based on pH and ionic strength values confirmed the formation of the oxidized form of Rtn (o-quinone) via laccase-like nanozyme activity of Cu-Guo NRs. A vivid and concentration-dependent color variation from green to dark yellow led to the visual detection of Rtn in a broad concentration range from 770 nM to 54.46 µM with a limit of detection (LOD) of 114 nM. The proposed methodology was successfully applied for the fast tracing of Rtn in the presence of certain common interfering species and various complex samples such as propolis dry extract, human biofluids, and dietary supplement tablets, with satisfactory precision. The sensitivity and selectivity of the developed sensor, which are bonuses in addition to rapid, on-site, cost-effective, and naked-eye determination of Rtn, hold great promise to provide technical support for routine analysis in the real world.
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