Optoelectronic Tweezers Micro‐Well System for Highly Efficient Single‐Cell Trapping, Dynamic Sorting, and Retrieval

Abstract Single‐cell arrays have emerged as a versatile method for executing single‐cell manipulations across an array of biological applications. In this paper, an innovative microfluidic platform is unveiled that utilizes optoelectronic tweezers (OETs) to array and sort individual cells at a flow rate of 20 µL min −1 . This platform is also adept at executing dielectrophoresis (DEP)‐based, light‐guided single‐cell retrievals from designated micro‐wells. This presents a compelling non‐contact method for the rapid and straightforward sorting of cells that are hard to distinguish. Within this system, cells are individually confined to micro‐wells, achieving an impressive high single‐cell capture rate exceeding 91.9%. The roles of illuminating patterns, flow velocities, and applied electrical voltages are delved into in enhancing the single‐cell capture rate. By integrating the OET system with the micro‐well arrays, the device showcases adaptability and a plethora of functions. It can concurrently trap and segregate specific cells, guided by their dielectric signatures. Experimental results, derived from a mixed sample of HepG2 and L‐O2 cells, reveal a sorting accuracy for L‐O2 cells surpassing 91%. Fluorescence markers allow for the identification of sequestered, fluorescence‐tagged HepG2 cells, which can subsequently be selectively released within the chip. This platform's rapidity in capturing and releasing individual cells augments its potential for future biological research and applications.