Lacticaseibacillus rhamnosusGG improves behavior, immune functions, and biological age markers in female mice with premature aging

Abstract Background Prematurely aged mice (PAM), characterized by an inadequate stress response, exhibit early immunosenescence and reduced lifespan compared with exceptional non‐PAM (E‐NPAM) of the same age. Lacticaseibacillus rhamnosus GG (LGG) has been proposed as a beneficial probiotic in healthy aging. This study aimed to evaluate the effects of LGG consumption over 2 and 4 weeks on behavioral parameters, peritoneal leukocyte function, and lifespan in adult female PAM. Results Female ICR‐CD1 mice were classified as either PAM or E‐NPAM and divided into three groups: (1) PAM control (PAMC), (2) PAM receiving LGG (PAMLGG), and (3) E‐NPAM control (E‐NPAMC). The PAMLGG group consumed a lyophilized LGG suspension in skim milk for 4 weeks, while the PAMC and E‐NPAMC groups received a skim milk solution. Behavioral tests were conducted after 2 weeks, and leukocyte function was analyzed after 2 and 4 weeks. The results showed that the PAMLGG group exhibited improved behavioral responses and enhanced immune functions, which was reflected in a lower biological age and in a longer longevity compared with the PAMC. Conclusion LGG supplementation positively influenced behavior, immune function, and biological age markers in female PAM, leading to increased longevity. These findings indicate that LGG administration could be a good nutritional strategy for slowing down the aging process, allowing a healthy longevity to be achieved. © 2025 Society of Chemical Industry.