Surface Modification of Biodegradable Polymer Enhances Liver Organoid Attachment and Integration Forming a Cholangiocyte Monolayer

Cell adhesion is a fundamental necessity for anchorage-dependent cells to thrive in the matrix. This process serves as the initial stage in a sequence of cellular activities, which includes cell diffusion, migration, proliferation, and differentiation. This study introduces a novel surface modification designed and engineered to enhance the attachment of cholangiocyte organoids and facilitate the spreading of cholangiocytes on the polymer surface. Our findings revealed that the recruiting of the collagen layer on the polydopamine (PDA) nanoparticle coating poly(lactide-co-trimethylene carbonate) (PLATMC) surface plays a key role in attracting the liver organoids and enhancing cell attachment and migration. This finding has promising results in generating a two-dimensional (2D) cholangiocyte monolayer through organoid attachment to the surface and subsequent cholangiocyte integration. The innovative approach combines surface modification with organoids culture technique, offering significant potential for advancing bile duct regenerative medicine and developing more complex three-dimensional (3D) tissues in vitro.