Development of aptamer-based monolithic sorbent in paper devices for lysozyme extraction in foods

BACKGROUND: Lysozyme (Lyz) is a protein commonly used as a food preservative, but it can cause severe allergic reactions. Detecting trace amounts of Lyz in food is essential, requiring sensitive, reliable, and accessible analytical methods. Aptamers (Apts) are recognition ligands that can be integrated into paper-based devices to offer low-cost and effective platforms for Lyz detection. However, the Apt-paper hybridization as sorptive phase remains insufficiently explored. RESULTS: ). Additionally, the developed Apt-monolith@paper was affordable (<4.5 €/device), stable, and reusable up to 5 extraction cycles. SIGNIFICANCE: This work presents, for the first time, an Apt-monolith@paper for Lyz extraction from food samples. This strategy paves the way for advancements in analyzing proteins or hazardous compounds in food, contributing to quality control and safety.