Preparation and Evaluation of RGD‐Conjugated Crosslinked PVA Tissue Engineered Vascular Scaffold with Endothelial Differentiation and Its Impact on Vascular Regeneration In Vivo

Abstract PVA has emerged as a prevalent material for the construction of vascular tissue engineering scaffolds. Nonetheless, the integration of 3D crosslinked polyvinyl alcohol (PVA) scaffolds featuring arginine‐glycine‐aspartate (RGD) binding remains a rarity in tissue engineering. In the present study, a PVA‐4‐azidobenzoic acid (AZ)‐RGD scaffold is prepared based on cross‐linking of two distinct PVA derivatives: one featuring photoreactive azides for ultraviolet (UV)‐crosslinking and the other incorporating RGD peptides. The results show that the PVA‐AZ‐RGD scaffold has good blood compatibility and biomechanical properties, with hydrophilic properties, and a hydrolysis rate of 27.31% at 12 weeks. Notably, the incorporation of RGD peptides significantly bolsters the attachment and proliferation of mesenchymal stem cells (MSCs) on the scaffolds, compared to non‐RGD‐conjugated controls. Furthermore, RGD conjugation markedly accelerates endothelialization of MSCs following 15 days of endothelial culture. Post‐transplantation, the PVA‐AZ‐RGD scaffold exhibits favorable blood flow patency, minimal immune rejection, promotes endothelialization and smooth muscle cell proliferation, and facilitates the development of extracellular matrix, ultimately contributing to the formation of regenerative artificial blood vessels. These comprehensive findings underscore the promising potential of RGD‐integrated, crosslinked PVA scaffolds for applications in vascular tissue engineering.