纳米孔
化学
基质(水族馆)
可穿戴计算机
检出限
膜
线性范围
电极
安培法
纳米技术
电化学
色谱法
材料科学
生物化学
计算机科学
有机化学
嵌入式系统
海洋学
物理化学
地质学
作者
Zeng-Qiang Wu,Xia Cao,Yu Huang,Chunmei Yu
标识
DOI:10.1021/acs.analchem.3c05216
摘要
Wearable sensors for non-invasive, real-time detection of sweat lactate have far-reaching implications in the fields of health care and exercise physiological responses. Here, we propose a wearable electrochemical sensor with gold nanoelectrode arrays fabricated on the nanoporous polycarbonate (PC) membrane by encapsulating lactate oxidase (LOx) in chitosan (CS) hydrogel for detecting body temperature and sweat lactate concurrently. Flexible gold nanoporous electrodes not only enhance electrode area but also offer a nanoconfined space to accelerate the catalytic reaction of LOx and control substrate concentration on the surface of LOx to decrease substrate inhibition. The proposed sensor has a long durability of 13 days and better selectivity for the detection of sweat lactate over a wide linear range (0.01–35 mM) with a low detection limit (0.144 μM). Furthermore, temperature-dependent transmembrane currents passing through the sensor are used to estimate body temperature. We then use multiple linear regression to adjust the effect of temperature on lactate detection and succeed in monitoring lactate molecules in sweat and body temperature during exercise.
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