黄瓜
转录因子
突变体
MYB公司
生物
细胞生物学
扩张素
转录组
基因表达
拟南芥
基因
遗传学
植物
作者
Chunhua Wang,Hui-Wen Yao,Chen Wang,Liyan Gao,Xiaoqian J. Chai,Kai Fang,Ying Du,Ning Hao,Jiangping Cao,Tao Wu
标识
DOI:10.1093/plphys/kiae140
摘要
The fruit neck is an important agronomic trait of cucumber (Cucumis sativus). However, the underlying genes and regulatory mechanisms involved in fruit neck development are poorly understood. We previously identified a cucumber yellow green peel (ygp) mutant, whose causal gene is MYB DOMAIN PROTEIN 36 (CsMYB36). This study showed that the ygp mutant exhibited a shortened fruit neck and repressed cell expansion in the fruit neck. Further functional analysis showed that CsMYB36 was also a target gene, and its expression was enriched in the fruit neck. Overexpression of CsMYB36 in the ygp mutant rescued shortened fruit necks. Furthermore, transcriptome analysis and reverse transcription quantitative polymerase chain reaction (RT-qPCR) assays revealed that CsMYB36 positively regulates the expression of an expansin-like A3 (CsEXLA3) in the fruit neck, which is essential for cell expansion. Yeast one-hybrid and dual-LUC assays revealed that CsMYB36 regulates fruit neck elongation by directly binding to the promoter of CsEXLA3. Collectively, these findings demonstrate that CsMYB36 is an important gene in the regulation of fruit neck length in cucumber plants.
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