Marinomonas transparens sp. nov. and Marinomonas sargassi sp. nov., isolated from marine alga

Two Gram-stain-negative, rod-shaped, non-spore-forming, strictly aerobic, motile bacteria with a single polar flagellum, designated strains C1424 T and C2222 T , were isolated from marine alga collected from the sea shore at Yantai, PR China. Strain C1424 T grew at 4–37 °C and in the presence of 1–9 % (w/v) NaCl, while strain C2222 T grew at 4–32 °C with 1–6 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences and concatenated amino acid sequences of 120 ubiquitous single-copy proteins showed that both strains C1424 T and C2222 T belonged to the genus Marinomonas , showing highest 16S rRNA gene sequence similarities to the type strains of Marinomonas primoryensis (98.1 %) and Marinomonas dokdonensis (98.1 %), respectively. The major fatty acids of the two strains were C 18 : 1 ω 6 c and/or C 18 : 1 ω 7 c , C 16 : 1 ω 6 c and/or C 16 : 1 ω 7 c and C 16 : 0 , their predominant polar lipids were phosphatidylethanolamine and phosphatidylglycerol, and their sole respiratory quinone was Q8. On the basis of polyphasic analyses, strains C1424 T and C2222 T are considered to represent two novel species within the genus Marinomonas , for which the names Marinomonas transparens sp. nov. and Marinomonas sargassi sp. nov. are proposed. The type strains are C1424 T (=KCTC 72119 T =MCCC 1K03601 T ) and C2222 T (=KCTC 72120 T =MCCC 1K03602 T ), respectively.