共轭体系
化学
恶唑啉
血红蛋白
试剂
红细胞
戊二醛
共价键
分子生物学
生物化学
生物
聚合物
色谱法
有机化学
催化作用
作者
Wataru Okamoto,Yuji Hiwatashi,Tatsuhiro Kobayashi,Yoshitsugu Morita,Hiroto Onozawa,Masayuki Iwazaki,Mitsutomo Kohno,Hirotaka Tomiyasu,Ryota Tochinai,Radostina Georgieva,Hans Bäumler,Teruyuki Komatsu
出处
期刊:ACS applied bio materials
[American Chemical Society]
日期:2023-07-28
标识
DOI:10.1021/acsabm.3c00392
摘要
Hemoglobin wrapped covalently with poly(2-ethyl-2-oxazoline)s (POx-Hb) is characterized physicochemically and physiologically as an artificial O2 carrier for use as a red blood cell (RBC) substitute. The POx-Hb is generated by linkage of porcine Hb surface-lysines to a sulfhydryl terminus of the POx derivative, with the average binding number of the polymers ascertained as 6. The POx-Hb shows moderately higher colloid osmotic activity and O2 affinity than the naked Hb. Human adult HbA conjugated with POx also possesses equivalent features and O2 binding properties. The POx-Hb solution exhibits good hemocompatibility, with no influence on the functions of platelets, granulocytes, and monocytes. Its circulation half-life in rats is 14 times longer than that of naked Hb. Hemorrhagic shock in rats is relieved sufficiently by infusion of the POx-Hb solution, as revealed by improvements of circulatory parameters. Serum biochemistry tests and histopathological observations indicate no acute toxicity or abnormality in the related organs. All results indicate that POx-Hb represents an attractive alternative for RBCs and a useful O2 therapeutic reagent in transfusion medicine.
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