农杆菌
生物
转化(遗传学)
基因工程
分生组织
接种
植物
生物技术
园艺
基因
开枪
遗传学
重组DNA
作者
Shuang Hao,Yongyan Zhang,Ruide Li,Pengyan Qu,Chunzhen Cheng
标识
DOI:10.1016/j.scienta.2023.112693
摘要
Agrobacterium-mediated genetic transformation is the leading technology for plant genetic engineering and gene functional analysis. Recently, various tissue culture-independent Agrobacterium-mediated in planta transformation (AMIPT) methods, such as floral dipping, seed inoculation, ovary/fruit injection and meristem/incision inoculation, have been successfully applied to numerous plant species. In this review, the current status and progress of the applications of AMIPT in horticultural plants and factors influencing its efficiency are described. Additionally, prospects for its future application in horticultural plants are presented. This will help to extend the use of AMIPT in a broader range of horticultural plant species for the purpose of genetic engineering and breeding.
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