协议(科学)
工作流程
计算机科学
可执行文件
灵活性(工程)
微生物群
计算生物学
数据科学
生物
生物信息学
数据库
操作系统
医学
统计
替代医学
数学
病理
作者
Liping Zeng,Bailong Zhang
摘要
Abstract Chromatin immunoprecipitation followed by sequencing (ChIP‐seq) is a widely employed technique for investigating protein‐DNA interactions. However, the absence of a standardized and clear workflow necessitates researchers to independently assemble methodologies from diverse resources. This lack of uniformity hampers reproducibility and makes version control a complex endeavor, thereby limiting the accessibility of ChIP‐seq analyses to individuals with extensive training in bioinformatics. In light of these challenges, we have developed an executable protocol that addresses these limitations. Our protocol encompasses all aspects of ChIP‐seq analysis, ranging from quality control of raw reads to peak calling and downstream functional analyses. We have implemented two distinct approaches for peak calling, providing researchers with flexibility to choose the most suitable method for their specific experimental needs. This protocol will contribute to the scientific community by providing a standardized and clear resource that will enhance the reproducibility and accessibility of ChIP‐seq analyses. © 2023 Wiley Periodicals LLC. Basic Protocol : ChIP‐seq analysis workflow Alternative Protocol : Call differentially enriched peaks by using MACS3
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