适体
宋内志贺氏菌
检出限
化学
色谱法
志贺氏菌
生物
生物化学
分子生物学
大肠杆菌
基因
作者
Yue He,Jianhe Wei,Peisheng Ma,Imran Mahmood Khan,Lili Zhang,Niazi Sobia,Yu Xia,Zhouping Wang
出处
期刊:Food Control
[Elsevier]
日期:2024-02-01
卷期号:156: 110111-110111
标识
DOI:10.1016/j.foodcont.2023.110111
摘要
Shigella Sonnei (S. sonnei) is a common foodborne pathogen causing intestinal diseases. In this study, a sandwich-structured colorimetric and surface-enhanced Raman scattering (SERS) dual methods adaptation sensor was prepared for the detection of S. sonnei. Firstly, two aptamers, SS4.1-2 (Kd = 43.5 ± 2.78 nM) and SP1-2.2 (Kd = 23.3 ± 3.01 nM) with good specificity and affinity were obtained by truncation strategy. Then, Fe3O4@SiO2@Ag nanoparticles were utilized to capture bacteria, while Cu-TCPP (Fe)/AuNPs nanosheets were used as signal probes due to their oxidase activity and SERS signals. The detection limits of the aptasensor were as low as 37 CFU/mL for the colorimetric signal and 7 CFU/mL for SERS signal. Furthermore, the aptasensor was successfully applied to detect S. sonnei in real samples with 94.95–112.92% and 98.40–103.95% recoveries for colorimetric and SERS methods, respectively. A more rapid and accurate method to detect S. sonnei contamination in food was improved.
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