Sera samples (575) were collected from 17 breeds of apparently healthy dogs from different parts of the country and tested for the presence of antibodies against cytotoxin-I antigen ELISA from S. Weltevreden (BM1613) to know the seroprevalence of salmonellosis. The overall seroprevalence was 58.08%. Place-wise the highest seroprevalence was in sera samples collected from Dehradun (92.00%), while breed-wise maximum sera samples of Labrador (81.58%) showed the presence of anti- cytotoxin-I antibodies of salmonella. The difference of seroprevalence of salmonellosis between 2 sexes was nonsignificant and the age group of 7–9 years and above showed the highest serprevalence (63.37%). Our results showed a high seroprevalence of salmonellosis in dogs, which indicates a serious threat to human health.