OBJECTIVE: To study the association between dietary glucose response and dietary fiber through evaluating dietary glycemic index (DGI) and diet glycemic load (DGL). METHODS: Dietary intakes of 105 subjects with diabetes mellitus were measured by means of two 3-day food records through weighting. DGI and DGL were calculated according to food glycemic index and carbohydrate intakes. The content of total, insoluble and soluble dietary fiber were determined by enzymatic-gravimetric method. RESULTS: The values of DGI and DGL were 62.9 +/- 6.8 and 142.4 +/- 39.8 respectively. The intakes of TDF, IDF and SDF were (22.5 +/- 6.7), (16.1 +/- 5.5), (6.4 +/- 2.0) g/d respectively. The correlation coefficient between DGI and TDF were -0.407 (P < 0.01). When compared with the subjects with lowest DGI, the intakes of vegetable foods, TDF, IDF and SDF in subjects with highest DGI were decreased by 221.9 g, 6.5 g, 5.0 g, 1.4 g respectively (P < 0.01), and that of cereal foods were increased by 68.0 g (P < 0.01). Of the different sources of fiber, vegetable fiber was most strongly decreased. CONCLUSION: DGI was negtively related to TDF intakes. The diet rich in vegetables and dietary fiber may decrease the dietary glucose response.