Chemical Constituents from the Rhizomes of Curcuma kwangsiensis

Abstract Aim To study the bioactive constituents of Curcuma kwangsiensis . Methods The compounds were isolated by silica gel, Sephadex LH-20, and Rp-18 gel column chromatography, and preparative HPLC. The structures were elucidated by extensive spectroscopic methods including 2D-NMR and HRESI-MS. Results Four new compounds, methyl p -(1-β- d -glucopyranosyloxy-1-methylethyl) benzoate ( 1 ), p -(1-β- d -glucopyranosyloxy-1-methylethyl) benzoic acid ( 2 ), 2, 3, 5-trihydroxy-1-(3-methoxy-4-hydroxyphenyl)-7-(3, 5-dimethoxy-4-hydroxyphenyl) heptane ( 3 ), and 2, 3, 5-trihydroxy-1-(4-hydroxyphenyl)-7-(3, 5-dimethoxy-4-hydroxyphenyl) heptane ( 4 ), and three known ones, 1, 5-epoxy-3α-hydroxy-1-(3, 4-dihydroxy-5-methoxy-phenyl)-7-(3, 4-dihydroxy) heptane ( 5 ), (3 R ′, 5 S ′)-3, 5-dihydroxy-1-(4-hydroxy-3-methoxyphenyl)-7-(3, 4-dihydroxyphenyl) heptane ( 6 ), and 2, 4, 6-trihydroxyacetophenone-2, 4-di- O -β- d -glucopyranoside ( 7 ), were isolated. Conclusion Compounds 1–4 were new natural compounds, and 3 – 7 showed strong inhibitory effects on NO production induced by LPS in macrophages.