降级(电信)
土霉素
环境化学
化学
环境科学
地质学
生物化学
抗生素
工程类
电信
作者
Raphaël Delépée,Daniel Maume,Bruno Le Bizec,Hervé Pouliquen
出处
期刊:Journal of Chromatography B: Biomedical Sciences and Applications
[Elsevier]
日期:2000-10-01
卷期号:748 (2): 369-381
被引量:56
标识
DOI:10.1016/s0378-4347(00)00355-8
摘要
A very specific high-performance liquid chromatography-mass spectrometric method for the determination of natural tetracyclines was developed in order to characterise the degradation products of oxytetracycline in sediments. First, extraction used a clean up step with a Bond Elut Certify LRC cartridge. A 3 microm Spherisorb ODS1 column was then used with a methanol, acetonitrile and oxalic acid mobile phase gradient. Chromatographic resolution in these conditions was 3.31 between oxytetracycline and tetracycline. Two liquid chromatography-mass spectrometry methodologies based on a particle beam and a frit fast atom bombardment interface were developed. In the first approach, ionisation was performed in the negative chemical mode using methane as reacting gas. In the other case, glycerol-thioglycerol mixture was used as matrix to ensure good sensitivity. MS-MS experiment was performed to determinate oxytetracycline fragmentation pattern in the perspective of degradation product study.
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