ConclusionIn our study, negative cultures were 60,6%, perhaps because of concurrent antimicrobial therapy or poor clinical evaluation. In this work, Proteus mirabilis is the major pathogen, followed by E. coli, probably because Proteus mirabilis is commonly associated with recurrent urinary tract infections in dogs thus providing easily detectable clinical signs (haematuria).Susceptibility testing revealed diversity in resistance patterns and phenotypes in the different genera of uropathogens, which corroborates the importance of urine culture and bacterial identification in the diagnosis and treatment of urinary tract infection in dogs.