The results of bioassays showed that the larvae of Spodoptera litura(Fabricius)fed with different genotypes of soybean had remarkable differences in susceptibility to beta-cypermethrin. Compared with the larvae fed with highly susceptible, susceptible and mediate resistant soybean varieties, the larvae fed with resistant and highly resistant soybean varieties were more sensitive to beta-cypermethrin. The larvae fed with all different genotypes of soybean had little differences in susceptibility to phoxim, LC_(50) were 3.98-7.66 mg/L,except the highly resistant soybean variety,which was more sensitive to phoxim than that fed with other varieties,LC_(50) was 2.43 mg/L,the index of relative toxicity was 3. The larvae no matter fed with highly susceptible or highly resistant soybean varieties all had remarkable differences in susceptibility to beta-cypermethrin but little differences in susceptibility to phoxim by varying tested materials.