Characterizing the spatial distribution of dipeptides in rodent tissue using MALDI MS imaging with on-tissue derivatization

A highly efficient on-tissue derivatization method for the matrix-assisted laser desorption/ionization mass spectrometry imaging (MALDI MSI) of dipeptides in tissues has been developed via a combination of a home-built laser-assisted chemical transfer (LACT) technique and hexafluoroisopropanol (HFIP).