In-Silico Identification of New Genes in HIV-1 by ORF Prediction Method

In the present study the complete genome sequence of Human Immunodeficiency Virus-1 was searched and retrieved from Genbank database with accession number NC_001802.1. The sequence of HIV-1 applied for gene Identification by ORFs prediction method. ORF Finder revealed that total 39 ORFs were identified in all six possible reading frames (RF). Three ORFs were identified in +1 RF, four in +2RF, six in +3RF, six in -1RF, thirteen in -2RF, and seven in -3RF. The translated sequences of all identified ORFs were searched in NCBI nonredundant database using Protein BLAST. All ORFs found in +1, +2, +3RF, and ORF 4 in -1RF, ORF 10 in -2RF, ORF 2 and ORF 3 in -3RF were already identified by previous workers but the remaining ORFs in -1,-2, and -3 RFs, eleven different ORFs translations showed similarity with different protein sequences of different organisms in nonredundant protein sequence database.