细胞
细胞内
电池类型
细胞生物学
黄素组
化学
计算生物学
免疫系统
生物
生物化学
免疫学
酶
作者
Rob C. Oslund,Tamara Reyes‐Robles,Cory H. White,Jake H. Tomlinson,Kelly Crotty,Edward P. Bowman,Dan Chang,Vanessa M. Peterson,Lixia Li,Silvia Frutos,Miquel Vila-Perelló,David Vlerick,Karen D. Cromie,David H. Perlman,Sampat Ingale,Samantha D. O’ Hara,Lee R. Roberts,Grazia Piizzi,Erik C. Hett,Daria J. Hazuda,Olugbeminiyi O. Fadeyi
标识
DOI:10.1038/s41589-022-01044-0
摘要
The growing appreciation of immune cell-cell interactions within disease environments has led to extensive efforts to develop immunotherapies. However, characterizing complex cell-cell interfaces in high resolution remains challenging. Thus, technologies leveraging therapeutic-based modalities to profile intercellular environments offer opportunities to study cell-cell interactions with molecular-level insight. We introduce photocatalytic cell tagging (PhoTag) for interrogating cell-cell interactions using single-domain antibodies (VHHs) conjugated to photoactivatable flavin-based cofactors. Following irradiation with visible light, the flavin photocatalyst generates phenoxy radical tags for targeted labeling. Using this technology, we demonstrate selective synaptic labeling across the PD-1/PD-L1 axis in antigen-presenting cell-T cell systems. In combination with multiomics single-cell sequencing, we monitored interactions between peripheral blood mononuclear cells and Raji PD-L1 B cells, revealing differences in transient interactions with specific T cell subtypes. The utility of PhoTag in capturing cell-cell interactions will enable detailed profiling of intercellular communication across different biological systems.
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