赭曲霉毒素A
α生育酚
阿尔法(金融)
肾
赭曲霉毒素
食品科学
细胞培养
化学
生育酚
生物
抗氧化剂
生物化学
真菌毒素
遗传学
医学
维生素E
结构效度
护理部
患者满意度
作者
Hyun Jung Lee,Hae Dun Kim,Dojin Ryu
出处
期刊:Journal of Food Protection
[International Association for Food Protection]
日期:2023-05-01
卷期号:86 (5): 100082-100082
被引量:2
标识
DOI:10.1016/j.jfp.2023.100082
摘要
Food safety is a top priority for the protection of infants and young children. Ochratoxin A (OTA) is an emerging concern due to its high toxicity and occurrence in a wide range of agricultural crops and their derived food products including those foods and snacks destined for infants and young children. OTA is considered as a possible human carcinogen, and its main target organ is the kidney. The objective of this study was to investigate the protective effect of α-tocopherol against oxidative stress induced by OTA using human proximal tubule epithelial cells (HK-2). OTA showed dose-dependent increase in cytotoxicity (IC50 = 161 nM, p < 0.05) at 48 h, while treatment up to 2 mM α-tocopherol did not change cell viability. Levels of the reduced form of glutathione (GSH) were decreased with α-tocopherol treatment, although the ratio of the oxidative form (GSSG) to GSH remained the same. Among several genes associated with oxidative stress, expression of superoxide dismutase 1 (SOD1), catalase (CAT), glutathione reductase (GSR), and kidney injury molecule-1 (KIM-1) were significantly up-regulated by OTA treatment. CAT and GSR showed decreased expression at 0.5–2 mM α-tocopherol and OTA at IC50 value, KIM-1 was decreased at 0.5 mM α-tocopherol and OTA at IC50 value, and nuclear factor erythroid 2–related factor 2 (Nrf2) was decreased at 0.5–1 mM α-tocopherol and OTA at IC50 value. In addition, the levels of malondialdehyde (MDA) were increased significantly by OTA while significantly decreased by α-tocopherol. The results show that α-tocopherol may alleviate potential OTA-induced renal damage and oxidative stress through reducing cytotoxicity and enhancing the antioxidant defense systems.
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