Glass slide evanescent wave fluorescence biosensor integrated coupling aspherical lens with a sensing range of over 20 mm for multi‐wavelength operation

Abstract Evanescent wave fluorescence biosensor is a research hotspot in the field of biomedical detection due to high sensitivity and specificity. However, the fluorescence biosensor still shows small sensing range and high production cost. In this paper, a simple and cheap sub‐millimetre thickness glass slide evanescent wave fluorescence biosensor integrated an aspherical coupling lens is proposed. The proposed sub‐millimetre thickness glass slide fluorescence biosensor shows a sensing range of over 20 mm for multi‐wavelength operation for the three materials of N‐BK7, N‐LAF21 and N‐LASF41. This will be helpful for the evanescent wave fluorescence biosensor.