Green, simple analytical method for total biogenic amines content determination in wine using spectrophotometry

A simple, green and equitable procedure for total biogenic amines (BAs) content determination was developed. The scientific novelty lies in the use of commercially available S 0378 dye, the reaction of which with BAs results in a colour change of the solution. Sample preparation and analysis were simplified to make the method suitable for routine analyses even in resource-scarce settings. The optimization of the method was carried out using a Box-Behnken response surface design. The developed method has satisfactory figures of merit for putrescine equivalent determination with R2 in the range of 0.9906–0.9933 and recovery between 99.7 and 101.2 %. The method's greenness was assessed using AGREEprep. Finally, wine samples were analysed to demonstrate the applicability of the developed method.