From waste to resource: Metagenomics uncovers the molecular ecological resources for plastic degradation in estuaries of South China

Estuaries are hotspots of plastic pollution due to accumulated waste from surrounding rivers and coasts. However, the molecular ecological resources that possess plastic-degrading traits and their biogeographic distributions in estuarine waters remain to be elucidated. In this study, we mapped the distribution profiles of plastic-degrading genes (PDGs) in 30 subtropical estuaries in China based on metagenomic sequencing. A total of 41 PDG subtypes were observed in these estuaries. The Pearl River Estuary had higher diversity and abundance of PDGs than the east and west region estuaries. Genes for degrading synthetic heterochain and natural plastics were the most diverse and abundant types, respectively. The abundance of synthetic PDGs was significantly higher in estuaries affected by intense anthropogenic activities. Further binning strategies revealed diverse microbes with plastic-degrading ability in these estuaries. Rhodobacteraceae, a dominant plastic-degrading bacterial family, primarily carried PDGs for degrading natural plastics. Pseudomonas veronii carrying diverse PDGs was identified, which may be of value for further technical improvement of plastic degradation. In addition, phylogenetic and structural analyses of 19 putative 3HV dehydrogenases, the most diverse and abundant DPGs, showed inconsistent evolution with their hosts, but different sequences were conserved with consistent key functional amino acids. A potential biodegradation pathway for polyhydroxybutyrate by Rhodobacteraceae was proposed. The result implied that plastic-degrading functions are widely distributed in estuarine waters and metagenomics could be used as a promising screening tool for large-scale profiling of plastic-degrading potential in the natural environment. Our findings have important implications and provide potential molecular ecological resources for developing plastic waste removal technologies.