Controlled production of liposomes with novel microfluidic membrane emulsification for application of entrapping hydrophilic and lipophilic drugs

In view of the limitations in the reported methods for the preparation of liposomes, a new microfluidic membrane emulsification device was innovatively designed and fabricated to investigate the effects of prescription factors and process parameters on the formation of liposomes based on a liposome prescription suitable for encapsulating nucleotides. It was shown that precise control of liposome particle size can be achieved by adjusting prescription factors and process parameters, and the obtained liposomes have good stability. In this study, we efficiently encapsulated nicotinamide mononucleotide (NMN) and honokiol to achieve co-delivery and graded release of hydrophilic and hydrophobic drugs, and its inhibitory activity against colon cancer cells was significantly higher than that of the free drug. What's more, a new high-performance liquid chromatographic method was built up to determine the encapsulation rate of NMN liposomes, which would be useful for the development of NMN drug applications. Notably, we also extended the technique to drug delivery microsphere platform construction to achieve homogeneous and controllable preparation of high-viscosity calcium alginate microspheres. This study is a valuable guide for the construction of drug delivery system platforms and has promising applications.