Blautia parvula sp. nov., isolated from Japanese faecal samples

Two Gram-positive, anaerobic, non-spore-forming and coccoid or oval-shaped bacterial strains, namely, DN0138 T and DN0266, were isolated from faecal samples of healthy Japanese people. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain DN0138 T clustered with a species of the genus Blautia and was closely related to Blautia producta JCM 1471 T , Blautia coccoides JCM 1395 T , Blautia hominis KB1 T and ‘ Blautia marasmi ’ Marseille-P2377, with sequence similarities of 98.6, 98.5, 98.8 and 98.2 %, respectively. The average nucleotide identity values were 85.3 % for B. producta JCM 1471 T , 85.0 % for B. coccoides NCTC 11035 T , 84.3 % for B. hominis KB1 T and 84.3 % for ‘ B. marasmi ’ Marseille-P2377. The major end products of glucose metabolism were acetic acid, lactic acid and succinic acid. The genome length of strain DN0138 T was 6 247 046 bp with 46.7 mol% G+C content of genome sequence. Based on their phenotypic, cellular fatty acid and phylogenetic characteristics, the three isolates represent a novel species within the genus Blautia , for which the name Blautia parvula sp. nov. is proposed. The type strain is DN0138 T (=NBRC 113351 T =BCRC 81349 T ).