活力测定
碘化丙啶
纳米纤维
化学
替莫唑胺
吖啶橙
生物物理学
细胞
细胞凋亡
材料科学
纳米技术
癌症研究
程序性细胞死亡
生物化学
胶质母细胞瘤
医学
生物
作者
Melis Erçelik,Çağla Tekin,Fatma Nur Parın,Büşra Mutlu,Hülya Doğan,Gülçin Tezcan,Seçil Ak Aksoy,Mevlüt Gürbüz,Kenan Yıldırım,Ahmet Bekâr,Hasan Kocaelı,Mevlüt Özgür Taşkapılıoğlu,Pınar Eser Ocak,Berrin Tunca
标识
DOI:10.1016/j.ijbiomac.2023.126722
摘要
Glioblastoma (GB) has susceptibility to post-surgical recurrence. Therefore, local treatment methods are required against recurrent GB cells in the post-surgical area. In this study, we developed a nanofiber-based local therapy against GB cells using Oleuropein (OL), and rutin and their combinations with Temozolomide (TMZ). The polylactic acid (PLA) core-shell nanofiber webs were encapsulated with OL (PLAOL), rutin (PLArutin), and TMZ (PLATMZ) by an electrospinning process. A SEM visualized the morphology and the total immersion method determined the release characteristics of PLA webs. Real-time cell tracking analysis for cell growth, dual Acridine Orange/Propidium Iodide staining for cell viability, a scratch wound healing assay for migration capacity, and a sphere formation assay for tumor spheroid aggressiveness were used. All polymeric nanofiber webs had core-shell structures with an average diameter between 133 ± 30.7–139 ± 20.5 nm. All PLA webs promoted apoptotic cell death, suppressed cell migration, and spheres growth (p < 0.0001). PLAOL and PLATMZ suppressed GB cell viability with a controlled release that increased over 120 h, while PLArutin caused rapid cell inhibition (p < 0.0001). Collectively, our findings suggest that core-shell nano-webs could be a novel and effective therapeutic tool for the controlled release of OL and TMZ against recurrent GB cells.
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