细胞凋亡
A549电池
细胞周期
细胞生长
细胞毒性T细胞
化学
细胞色素c
生物
癌细胞
细胞培养
活性氧
细胞生物学
药理学
生物化学
癌症
体外
遗传学
作者
Misbah Tabassum,Bashir A. Lone,Mudasir Nazir Bhat,Anil Bhushan,Nagma Banjare,Esteban Manrique,Prasoon Gupta,Dilip M. Mondhe,Prem N. Gupta
出处
期刊:ACS omega
[American Chemical Society]
日期:2023-08-23
卷期号:8 (35): 31914-31927
标识
DOI:10.1021/acsomega.3c03649
摘要
Natural product-derived molecules exhibit potential as anticancer agents. Trilliumoside A, a new steroidal saponin, was obtained from rhizomes of Trillium govanianum, and its anticancer activity was investigated in the presented study. Trilliumoside A was investigated in a panel of cell lines, and it exhibited promising cytotoxic activity on the A549 cells (human lung cancer cells) with an IC50 of 1.83 μM. The mechanism of cell death induced by Trilliumoside A in A549 cells and its anticancer potential in murine tumor models (EAC and EAT) were presented in the current research. Trilliumoside A was found to induce apoptosis in A549 cells by increasing the expression of various apoptotic proteins, such as Bax, Puma, cytochrome C, cleaved PARP, and cleaved caspase 3. Additionally, Trilliumoside A regulates the expression of p53, CDK2, and Cyclin A by decreasing the mitochondrial membrane potential, elevating reactive oxygen species, and stopping the growth of A549 cells in the synthesis phase (S) of the cell cycle. Trilliumoside A showed a considerable reduction in the tumor volume, the amount of ascitic fluid, and the total cell number without affecting the body weight of animals. Our results demonstrate that Trilliumoside A inhibits the proliferation of human lung cancer cells by inducing DNA damage, arresting the cell cycle, and activating the mitochondrial signaling pathway. The study demonstrated the potential of Trilliumoside A as a potential anticancer agent.
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