脱氧核酶
化学
生物累积
检出限
串联质谱法
污染
环境化学
色谱法
质谱法
生态学
生物
作者
Hao Yang,Feng Li,Ting Xue,Rafal Kruszynski,Xuhan Xia,Rosa Busquets,Hong Gao,Yi Dong,Wenhu Zhou,Ruijie Deng
标识
DOI:10.1021/acs.analchem.2c04589
摘要
Lead contamination in the environment tends to enter the food chain and further into the human body, causing serious health issues. Herein, we proposed a Csm6-DNAzyme tandem assay (termed cDNAzyme) using CRISPR/Cas III-A Csm6 and GR-5 DNAzyme, enabling one-pot and sensitive detection of lead contamination. We found that Pb2+-activated GR-5 DNAzyme produced cleaved substrates that can serve as the activator of Csm6, and the Csm6-DNAzyme tandem improved the sensitivity for detecting Pb2+ by 6.1 times compared to the original GR-5 DNAzyme. Due to the high specificity of DNAzyme, the cDNAzyme assay can discriminate Pb2+ from other bivalent and trivalent interfering ions and allowed precise detection of Pb2+ in water and food samples. Particularly, the assay can achieve one-step, mix-and-read detection of Pb2+ at room temperature. We used the cDNAzyme assay to investigate the accumulation of lead in mice, and found that lead accumulated at higher levels in the colon and kidney compared to the liver, and most of the lead was excreted. The cDNAzyme assay is promising to serve as analytical tools for lead-associated environmental and biosafety issues.
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