A ternary nucleotide–lanthanide coordination nanoprobe for ratiometric fluorescence detection of ciprofloxacin

Abstract Ciprofloxacin (CIP) is a widely used broad‐spectrum antibiotic and has been associated with various side effects, making its accurate detection crucial for patient safety, drug quality compliance, and environmental and food safety. This study presents the development of a ternary nucleotide–lanthanide coordination nanoprobe, GMP‐Tb‐BDC (GMP: guanosine 5′‐monophosphate, BDC: 2‐amino‐1,4‐benzenedicarboxylic acid), for the sensitive and ratiometric detection of CIP. The GMP‐Tb‐BDC nanoprobe was constructed by incorporating the blue‐emissive ligand BDC into the Tb/GMP coordination polymers. Upon the addition of CIP, the fluorescence of terbium ion (Tb 3+ ) was significantly enhanced due to the coordination and fluorescence sensitization properties of CIP, while the emission of the BDC ligand remained unchanged. The nanoprobe demonstrated good linearity in the concentration range of 0–10 μM CIP. By leveraging mobile phone software to analyze the color signals, rapid on‐site analysis of CIP was achieved. Furthermore, the nanoprobe exhibited accurate analysis of CIP in actual drug and milk samples. This study showcases the potential of the GMP‐Tb‐BDC nanoprobe for practical applications in CIP detection.