角质形成细胞
角质层
表皮(动物学)
背景(考古学)
永生化细胞系
细胞生物学
细胞培养
皮肤当量
人体皮肤
生物
化学
解剖
遗传学
古生物学
作者
Gijs Rikken,Hanna Niehues,Ellen H. van den Bogaard
出处
期刊:Methods in molecular biology
日期:2020-01-01
卷期号:: 45-61
被引量:18
标识
DOI:10.1007/978-1-0716-0648-3_5
摘要
The three-dimensional culturing of human keratinocytes at the air–liquid interface yields a fully stratified epidermis including a functional stratum corneum and thus enables the study on epidermal structure and function in the context of biomedical, toxicological and pharmaceutical sciences. Here we provide a step-by-step detailed protocol for the isolation of human primary keratinocytes and the development of human epidermal equivalents generated from primary keratinocytes or immortalized keratinocytes (N/TERT-1; N/TERT-2G), including widely accepted procedures for the analysis of barrier function, tissue morphology, cell proliferation, and gene expression.
科研通智能强力驱动
Strongly Powered by AbleSci AI