Development of An ssDNA Aptamer-based Test Method for Chronic Myelogenous Leukemia K562 Cells

Objective To develop an ssDNA aptamer-based test method for chronic myelogenous leukemia(CML) K562 cells.Methods High specific ssDNA aptmers to K562 cells were obtained by Cell-SELEX(Systematic evolution of ligands by exponential enrichment) technique,based on which a specific test method for K562 cells was developed by combination of double point binding assay and biotin-streptavidin bead technique,and verified for specificity and sensitivity.Results K562,HL-60 and control cells were tested by the developed method separately.The fluorescence intensity of K562 cells was significantly different from those of HL-60 and control cells(P 0.01),while showed no significant difference in HL-60 and control cells(P 0.05),indicating high specificity of the developed method.Less than 100 K562 cells were detected by the developed method,indicating high sensitivity.Conclusion A novel ssDNA aptamer-based test method for CML K562 cells was successfully developed,which provided a new tool for molecular diagnosis of CML and a new route to separation of surface substance of K562 cells.