Influence of TDZ and BA on efficiency of plant regeneration via organogenesis in soybean

The embryonic axes of immature seeds and spouting seeds were induced organogenesis on different induction media containing TDZ, BA. The regenerated plants via organogenesis were obtained and the effects of plant growth regulator and genotypes were analyzed. Multiple shoots were induced from embryonic axes of all genotypes cultured on all induction media containing TDZ. TDZ could induce the organogenesis of embryonic axes of immature seeds in soybean. High efficiency of organogenesis appeared when the embryonic axes were induced on the media containing 0.75mg/L TDZ combined with 2.0mg/L BA. The media contained higher concentration of NAA could increase the efficiency of organogenesis in soybeans.