Determination of Chlorophyll a in the Surface Sea Water with HPLC

A HPLC method has been used for determination of chlorophyll-a in the surface sea water of the Liaodong Bay. ODS-2 Spherisorb C18 reversed phase column is used. UV detection wavelenth is 436 nm. The HPLC solvents are methanol, 0.5 mol/L ammonium acetate aaq., acetonitril, water and ethyl acetate. They are all of HPLC-grade. Limit of detection is 10 ng/L. The correlations of three methods (spectrometer, fluorescence, HPLC) are compared.