The Effect of TGF-β3 on the Melanocyte Cultured in vitro of Alpaca

The aim of this study was to investigate the effect of TGF-β3on the phenotype of alpaca melanocyte cultured in vitro.The melanocyte proliferation,gene expression of MITF,TYR and TYRP2 at the transcript and translation level were detected and melanin production was analyzed after the addition of TGF-β3with different concentrations of 6.25,12.5,25,50ng·mL-1in melanocyte medium.The results showed that:Compared to the normal melanocyte,the proliferation of melanocytes was inhibited within 30 hby the addition of 50ng·mL-1 TFG-β3and then the amount of cells could be maintained without dependence on the concentration of TGF-β3after 30h;MITF,TYR and TYRP2 expression were down-regulated and melanin production also reduced after the addition of TGF-β3.The dose of 50ng·mL-1 TGF-β3caused more significant effects.The results suggested that TGF-β3had the important effects on the biology of alpaca melanocyte by regulating the expression of MITF,TYR,TYRP2 and melanin production.