Effect of vacuum treatment on secondary structure of superoxide dismutase investigated by circular dichroism spectrum

The time effects of different vacuum treatment on secondary structure of superoxide dismutase(SOD) were investigated experimentally via the circular dichroism(CD) spectrum.The results revealed that the different vacuum treating time(3~15min) affects the relative contents of α-helix,β-sheet,β-turn and random coil of SOD differently.As compared with the contrast,the relative contents of α-helix,β-turn and random coil decrease to 26.1%~41.3%,52.1%~68.8% and 2.1%~3.8% respectively,while the β-sheet content increased by 6.5%~9.2%.This indicated that the vacuum treatment tends to transform the α-helix,β-turn and random coil into β-sheet of the SOD.