Effects of vipral acid combined with basic fibroblast growth factor on proliferation of neural stem cell

Objective To investigate the effects of vipral acid(VPA) combined with basic fibroblast growth factor(bFGF) on proliferation of neural stem cell.Methods VPA and bFGF were used to the neural condition culture media of neural stem cells(NSCs) isolated and cultured from hippocampus tissue of brain in E15(embryonic stages) SD rat.Three treated groups were VPA+bFGF group,bFGF group and VPA group.The neural clone's numbers,diameter and morphology was observed.Results At the first day of primary culture,the number of NSCs clone in VPA+bFGF group was a little more than other groups,and no disparity was observed among different groups.At the 3rd day,the number of NSCs clone in VPA+bFGF group iwas much more than that of other two groups.and the diameter of NSCs in VPA+bFGF group was 2 times than that of other groups.The diameter of NSCs in VPA+bFGF group was 1.5 times as that of other groups at 7th and 10th day.Conclusion VPA enhances the promotional effect of bFGF on the proliferation of NSCs.