RP-HPLC simultaneous determination of five major active components in Jinlingzi decoction

Objective:To establish the method for determination of protopine,coptisine,palmatine,tetrahydropalmatine and toosendanin in Jinlingzi decoction.Methods: RP-HPLC separation was carried on a Kromasil C18(250 mm×4.6 mm,5 μm) column with the mobile phase consisting of acetonitrile and 0.05% acetic acid(adjusted to pH 6.0 with ammonia) in a programme of gradient elution.The flow rate was 0.8 mL·min-1 with a column temperature at 30 ℃,and the detection wavelength was set at 230 nm.Results: The linear ranges of protopine,coptisine,palmatine hydrochloride,tetrahydropalmatine and toosendanin were 1.76-35.2,1.84-36.8,2.20-44.0,2.24-44.8,and 8.48-63.6 μg·mL-1;The average recoveries(n=6) for the sample preparation of the markers were 103.5%,101.2%,102.7%,100.9%,97.1%,respectively.Conclusion: The developed method is simple,accurate and feasible,it is a suitable for the determination of protopine,coptisine,palmatine,tetrahydropalmatine,and toosendanin in Jinlingzi decoction.Otherwise it gives a comprehensive and objective evaluation for Jinlingzi decoction.