胞间连丝
生物
运动蛋白
突变体
细胞内
番茄斑萎病毒属
细胞生物学
过敏反应
病毒学
烟草花叶病毒
病毒
植物病毒
基因
遗传学
核糖核酸
植物抗病性
番茄斑点萎蔫病毒
细胞质
外壳蛋白
作者
Wenyang Zhao,Lei Jiang,Zhike Feng,Xiaojiao Chen,Ying Huang,Xue Fan,Changjun Huang,Yong Liu,Ли Фан,Yating Liu,Xiaorong Tao
摘要
The movement protein NSm of Tomato spotted wilt tospovirus (TSWV) plays pivotal roles in viral intercellular trafficking. Recently, the TSWV NSm was also identified as an avirulence (Avr) determinant during the Sw-5b-mediated hypersensitive response (HR). However, whether the cell-to-cell movement of NSm is coupled to its function in HR induction remains obscure. Here, we showed that the NSm mutants defective in targeting plasmodesmata and cell-to-cell movement were still capable of inducing Sw-5b-mediated HR. In addition, introduction of a single amino-acid substitution, C118Y or T120N, identified previously from TSWV resistance-breaking isolates, into the movement-defective NSm mutants resulted in the failure of HR induction. Collectively, our results showed that the intercellular trafficking of NSm is uncoupled from its function in HR induction. These findings shed light on the evolutionary mechanism of R-Avr recognition and may be used to explain why this uncoupled phenomenon can be observed in many different viruses.
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