肝细胞癌
病毒学
乙型肝炎病毒
生物
病毒
细胞培养
肝炎
丙型肝炎病毒
抗原
乙型肝炎病毒DNA聚合酶
病毒转化
乙型肝炎
免疫学
癌症研究
遗传学
作者
Kiyotaka Fujise,Seishi Nagamori,Satoshi Hasumura,Sadamu Homma,H Sujino,Tomokazu Matsuura,Kyoko Shimizu,Minoru Niiya,H Kameda,Kohei Fujita
出处
期刊:PubMed
日期:1990-10-01
卷期号:37 (5): 457-60
被引量:19
摘要
The authors successfully established 5 hepatocellular carcinoma cell lines, JHH-1, 2, 4, 5 and 6, derived from hepatitis B surface antigen seronegative patients and one line. JHH-7, from a patient considered to be a hepatitis B virus carrier. In the culture media of any of the JHH cell lines, including JHH-7, hepatitis B surface antigen was not detected by radioimmunoassay. However, in JHH-7, integration of hepatitis B virus DNA was confirmed at two sites on the chromosomes of this line by Southern blot hybridization. In contrast, in other JHH cell lines derived from hepatitis B surface antigen seronegative patients, integration of hepatitis B virus DNA into the chromosomes of cells was not detected. These cell lines will be useful in the investigation of hepatocellular carcinoma development, especially for research into non-A/non-B hepatitis viruses.
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