Summary This study describes the development of a fast and accurate method to determine the triacylglycerols ( TAG s) content in lipids fraction. It comprises a solid phase chromatographic separation and a use of a commercially available kit followed by spectrophotometric determination. Evaluations of TAG s contents in heated edible oils (canola oil and sesame oil) by the new methods were good agreements with those by the conventional open column method. Furthermore, this method was applied to estimate TAG s contents in the lipids from rice grains with different polishing levels.