Sesquiterpenoids with anti-neuroinflammatory effects in vitro from Laggera alata

A total of four sesquiterpenoids were isolated and identified from Laggera alata . Compounds 1 and 2 were previously undescribed compounds. The absolute configuration of compounds 3 and 4 were deduced for the first time. The new compounds were characterized using HRESIMS NMR, and ECD methods. They were (2 S ,4 R ,5 S ,7 S )-3-methoxy-arimophane-1(10),11(13)-diene-12-acid ( 1 ) and (7 S ,10 S )-eudesmane-4,11(13)-diene-3-one-12-acid ( 2 ). All compounds were evaluated for their anti-neuroinflammatory activities on LPS-induced BV2 cells. Compound 2 exhibited significant anti-neuroinflammatory activity at concentrations of 10, 20, and 40 μ M. Only compounds 1 , 3 , and 4 showed anti-neuroinflammatory activity at a concentration of 40 μ M. These results confirm that L. alata contains sesquiterpenoids, which demonstrate potential anti-neuroinflammatory properties, thereby establishing a pharmacological foundation for combating neuroinflammation. • Compounds 1 and 2 were previously undescribed compounds. • The absolute configuration of compounds 3 and 4 were deduced for the first time. • Compounds 1 - 4 exhibited significant anti-neuroinflammatory activity at a concentration of 40 μ M.