海马结构
伊诺斯
海马体
NADPH脱氢酶
一氧化氮合酶
突触可塑性
嗅球
小脑
多聚甲醛
化学
神经科学
生物
锥体细胞
长时程增强
一氧化氮
生物化学
中枢神经系统
内分泌学
受体
有机化学
作者
Jay L. Dinerman,Ted M. Dawson,Michael J. Schell,Adele M. Snowman,Solomon H. Snyder
标识
DOI:10.1073/pnas.91.10.4214
摘要
Using antibodies that react selectively with peptide sequences unique to endothelial nitric oxide synthase (eNOS), we demonstrate localizations to neuronal populations in the brain. In some brain regions, such as the cerebellum and olfactory bulb, eNOS and neuronal NOS (nNOS) occur in the same cell populations, though in differing proportions. In the hippocampus, localizations of the two enzymes are strikingly different, with eNOS more concentrated in hippocampal pyramidal cells than in any other brain area, whereas nNOS is restricted to occasional interneurons. In many brain regions NADPH diaphorase staining reflects NOS catalytic activity. Hippocampal pyramidal cells do not stain for diaphorase with conventional paraformaldehyde fixation but stain robustly with glutaraldehyde fixatives, presumably reflecting eNOS catalytic activity. eNOS in hippocampal pyramidal cells may generate the NO that has been postulated as a retrograde messenger of long-term potentiation.
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