质粒
化学
洗脱
DNA
色谱法
离子交换
高效液相色谱法
DNA超螺旋
选择性
离子
催化作用
生物化学
有机化学
DNA复制
作者
Clara R. Smith,Randolph B. DePrince,Jennifer Dackor,Debra Weigl,Jack D. Griffith,M. Persmark
标识
DOI:10.1016/j.jchromb.2007.04.005
摘要
We sought to establish a single anion-exchange HPLC method for the separation of linear, open circular and supercoiled plasmid topoisomers using purified topoisomeric forms of three plasmids (3.0, 5.5 and 7.6 kb). However, finding one condition proved elusive as the topoisomer elution order was determined to depend on salt gradient slope. The observed change in selectivity increased with plasmid size and was most pronounced for the linear form. Indeed, the elution order of the linear 7.6 kb plasmid was reversed relative to the supercoiled form. This observation may have implications for methods used in quality control of plasmid DNA.
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