Preparative methods for disk electrophoresis with special reference to the isolation of pituitary hormones

The design and use of several devices which can be used for the isolation of proteins by the disk electrophoretic technique are described. The methods include: means of accurately and reproducibly sectioning columns of polyacrylamide; electrophoretic elution of protein from gel sections; and a preparative device for collecting protein components as they emerge from the end of an electrophoretic column.