布鲁氏菌病
小肠结肠炎耶尔森菌
血清学
耶尔森尼亚
布鲁氏菌
生物
爆发
牧群
布鲁氏菌科
抗体
耶尔森菌病
病毒学
微生物学
免疫学
布鲁氏菌
细菌
肠杆菌科
大肠杆菌
动物科学
生物化学
遗传学
基因
作者
Vincent Weynants,Anne Tibor,Philippe Denoël,Claude Saegerman,Jacques Godfroid,P. Thiange,Jean‐Jacques Letesson
标识
DOI:10.1016/0378-1135(95)00153-0
摘要
During the last four years, an increasing number of cattle herds were classified positive by brucellosis screening tests in areas of Belgium and France free of the disease. No clinical symptom of brucellosis was reported in these animals and no Brucella abortus strains were isolated. After two years, no brucellosis outbreak was registered in all of the herds concerned. On this basis, all the serological reactions observed were classified as false positive. An ELISA using Yersinia Outer membrane Proteins (YOPs) as antigens was developed in order to discriminate between a Yersinia enterocolitica O:9 infection and a Brucella abortus infection. Antibodies against YOPs were detected in sera from Y. enterocolitica O:9 experimentally infected cattle (n = 4) but not in sera from B. abortus experimentally infected cattle (n = 4). In a field study, 66.7% of the 174 serum samples from cattle presenting false positive serological reactions showed anti-YOPs antibodies whereas only 10% of 454 sera, classified negative by the brucellosis screening tests, showed anti-YOPs antibodies. Our results suggest that infections with Y. enterocolitica O:9 may cause false positive reactions in brucellosis testing.
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