Amplified detection of DNA by an analyte-induced Y-shaped junction probe assembly followed with a nicking endonuclease-mediated autocatalytic recycling process

A simple, isothermal and autocatalytic system for the amplified detection of DNA was ingeniously designed based on target-triggered Y-shaped junction probe assembly, followed with a nicking endonuclease-mediated cascade recycling process. A superior detection limit of 0.1 pM towards the target DNA with an excellent selectivity could be achieved.